Hla typing was assigned using the software hla visual 2. Luminex labscan 100 multiplexing cyometry analyzer andbio. The system delivers fast and costeffective bioassay results on many assay formats including nucleic acid assays, receptorligand assays, immunoassays, and enzymatic assays. The first is xmap microspheres, a family of 100 fluorescently dyed 5. The systems xponent software is designed for protocolbased data acquisition with robust data regression analysis. Sales and support for magpix, 100200, and flexmap 3d luminex instrument systems involving detection. This information is presented to support researchers who use magpix, 100200, and flexmap 3d luminex systems. Milliplex kits are based on the luminex xmap beadbased. The second is a flow cytometrybased instrument, the luminex 100200 analyzer, which. This version of labscan was developed by a team of top researchers from the swiss. The labscan3d and labscan 100 systems are based on luminex xmap technology and use luminex xponent software for data acquisition. Use of belysa immunoassay curve fitting software affords the user.
The mixed assay detects the presence of antibody to class i andor class ii hla antigens. Luminex 100200 sets the standard for multiplexing, with over 11,000 units sold globally. I and ii dna typing protocols using one lambda labtype sso kits with luminex xmap microsphere array technology, labscan 100 flow analyzer, and labtype software for hla allele assignments. Conduct immunoassays, nucleic acid research, receptorligands studies and enzymatic research all with the luminex 100. Please select either a category andor a product line before searching for documents. The luminex 100 200 system is a flexible analyzer based on the principles of flow cytometry.
The products utilized were labscreen mixed kit one lambda, ca, usa and labscreen pra. One lambdas state of the art equipment provides optimal support for high and low throughput laboratories. Labtype hd uses the luminex based labscan 100 or lab scan3d platform to deliver rapid results and superior resolution. When used with the labscreen and labtype family of products, assignment of hla antibodies as well as hla typing is possible with the additional analysis support of hla fusion. Except as specified in the end user license agreement, any reproduction. Heres how to start up the luminex 200 instrument 1. This summary of 510k safety and effectiveness information is being submitted in accordance. Conduct immunoassays, nucleic acid research, receptorligands studies and enzymatic research. The luminex 100 is software uses the videosoft vsflexgrid pro 7. By multiplexing up to 100 assays in a single well, the luminex 100 can dramatically increase the amount of information generated from precious samples. The luminex 100200 analyzer integrates key xmap detection components such as lasers, optics, fluidics and highspeed digital signal processors.
Luminex corporation 100 liquid array analyzer system with. May, 2015 the luminex 100200 system is the combination of three core xmap technologies. Biotinylated detection antibodies specific to the analytes of interest are added and form an antibodyantigen sandwich. Samples were analyzed using the labscan 100 and the labscan 100 luminex xy platform. Labscan 100 is using luminex xmap technology, state of the art optics, electronics, and software the labscan 100 is a sensitive flow analyzer capable of performing up to 100 assays simultaneously. The luminex 100200 system is a clinical diagnostics instrument that allows multiplexing. Luminex is a leading developer and provider of mainframe data solutions and technologies, including luminex mainframe virtual tape and luminex mainframe data integration solutions. Determines percent pra and identifies antibody specificities using a panel of colorcoded microbeads coated with hla antigens purified from different cells. If your luminex instrument needs attention or you require. The luminex 100200 system is the combination of three core xmap technologies. Labtype hd high definition typing labtype hd is a premier technology in our sso molecular prod uct line. Turn on the luminex 200 analyzer, xy platform, and, if used, the luminex sheath delivery system. Mar 01, 20 this latest upgrade to the hla fusion software line provides all the tools and applications from version 2. Except as specified in the end user license agreement, any reproduction in whole or in part is strictly prohibited.
Using the labscan systems with our labtype and labscreen family of products allows the user to make assignments of hla antibodies as well as hla typing with the analysis support of hla fusion. The sample is added to a mixture of colorcoded beads, precoated with analytespecific capture antibodies. Luminex 100 suspension array immunoassay analyzer xmap technology system with software. State of the art optics, electronics, and software make the labscan systems capable of rapidly processing up to 96 samples. The contents of this manual and the associated luminex software are the property of luminex and are ed. The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. The luminex 100200 system is the combination of three core. It delivers fast and costeffective bioassay results on many assay formats including nucleic acid assays, receptorligand assays, immunoassays, and enzymatic assays.
This latest upgrade to the hla fusion software line provides all the tools and applications from version 2. The resulting mixtures were incubated for 30 min at room temperature and protected from light. The determination of positive and negative sera was performed with one lambda software labscreen pra software, one lambda according to the manufacturers protocol. Use with our labtype and labscreen family of products to assign percent pra, determine hla antibody and make hla class i and class ii allele assignments for tissue typing. With its ability to process 500 bead regions simultaneously, there is a significant increase in the size of screenable analytes. The acquisition on a labscan 100 by luminex software can then be performed providing means of fluorescence mfi for each bead.
The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as. Utilizes the labscan 100 or labscan3d for analysis of up to 100 or 500 bead regions, respectively, in a single test. The luminex 200 detects up to 100 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Production and characterization of chimeric antihla. Separately available analysis software hla fusion can. Readeranalyzers one lambdas state of the art equipment provides optimal support for high and low throughput laboratories. The labscan3d and labscan 100 systems are based on luminex xmap technology. Jan 15, 2018 heres how to start up the luminex 200 instrument 1. Masterplex, starstation, liquichip, bioplex manager, labscan 100 would need to follow instrument. Both bead types are internally dyed with precise proportions of red and infrared fluorophores. When used with the labscreen and labtype family of. Antibody screening and identification of antibody specificity were carried out using a labscan 100 flow analyser luminex corporation, texas, usa.
Labscan3d multiplex flow analyze delta laboratory co. Operated perkinelmer 9600 and 9700 thermocyclers, and labscan 100 luminex flow analyzers. Mar 30, 2007 antibody screening and identification of antibody specificity were carried out using a labscan 100 flow analyser luminex corporation, texas, usa. The luminex system is the combination of three core xmap technologies.
Class i and class ii assays may be tested separately or together. The pra tests can detect antibodies and their specificities against the hla antigens in each labscreen. Labscan is an easy to use software application specifically designed for acquiring images from twodimensional electrophoresis gels and blots, using the imagescanner. The luminex labscan 100 system enables you to multiplex simultaneously measure up to 100 analytes in a single microplate well, using very small samples. The luminex 200 multiplex reader is a clinical diagnostics instrument that allows multiplexing of up to 100 analytes in a single well of a microtiter plate, using very small samples. The system enables you to multiplex simultaneously measure up to 100 analytes in a single microplate well, using very small samples.
When used with one lambdas labscreen or labtype products, this system can assign percent pra, determine hla antibody and make hla class i. Labscan 100 luminex100200 or labscan3d luminexflexmap 3d for analysis of up to 100 or 500 bead regions, respectively, in a single test. The luminex 100200 system is a clinical diagnostics instrument that allows. The third component is the xponent software, which is designed for. Luminex fluoro analyzer the most advanced luminex system to date. Posted on august 7, 2014 november 24, 2015 by luminex corporation. Luminex technology for molecular typing and both are. Luminex corporation 100 liquid array analyzer system with xyp. Luminex 100200 for life science research standard for. Clinical diagnostics instruments luminex 100200 system. The ability to use either magnetic or polystyrene beads makes this instrument a popular choice for laboratories needing greater flexibility. Lx7081402 multiplexing up to 100 analytes per sample 80 on magplex microspheres dynamic range typical. This information is presented to support researchers who use magpix, 100 200, and flexmap 3d luminex systems.
The microbead fluorescent emission of rphycoerythrin was then detected and quantified using the labscan 100 flow analyzer one lambda. Luminex labscan 100 multiplexing cyometry analyzer system. Automated rssop hla typing development and validation. The labscan3d and labscan 100 are built on the proven luminex xponent software for data acquisition and analysis.
897 1255 39 997 154 212 29 1237 185 1440 1469 738 777 862 616 417 1199 427 95 230 1218 507 1412 112 891 1469 1182 1220 310 438 1466