Interferongamma elisa protocol coat plate with 50ul of a 2. Ifn gamma plays a major role in innate and adaptive immunity by promoting t cell differentiation into th1 and the development of treg, as well as by inhibiting t cell differentiation into th17. Human ifn gamma elisa kit ab174443 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifn gamma protein in human cell culture supernatant, plasma and serum samples. Supernatants from the samples were used for protein analysis. Human ifn alphabeta r2 elisa kit ifnar2 ab264610 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifn alphabeta r2 ifnar2 protein in hep plasma, serum, and cell lysate. The human interferon beta ifn beta elisa kit accurately measures natural human interferon beta. Includes elisa plates and reagents for 480 tests 5 x 96 wells our research use assay uses two different mouse anti bovine interferon gamma ifn.
Results obtained for naturally occurring human ifn gamma samples showed. The interaction of ifn alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. Results obtained for naturally occurring human ifn gamma samples showed linear curves that were parallel to the standard. Sets, in two appealing formats, address the need for sensitivity. Mouse interferon alpha 2 elisa kit ab211648 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 2 protein in cell culture extracts, cell. Sets, in two appealing formats, address the need for.
It uses our proprietary simplestep elisa technology. Mouse ifn gamma uncoated elisa components of 10plates. Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. Elisa for natural or recombinant human ifng in serum, plasma, and cell culture supernatants. Mbs2512904 96t48t24t this manual must be read attentively and completely before using this product. Peg interferon alpha2a pegasys 45 microgram subcutaneously weekly peg interferon alpha2b is not recommended due to lack of experience. Mouse ifn no crossreactivity detected with rat ifn. The assay will exclusively recognize both natural and recombinant ms ifn principle of the method. Elisa technical guide and protocols thermo fisher scientific. Elisa kit for cell and tissue lysates sigmaaldrich.
Mix all reagents of the human interferon gamma ifn. Human ifn alpha interferon alpha elisa kit elisa genie. The opteia set for mouse interferon g ifn g contains the components necessary to develop enzymelinked immunosorbent assays elisa for natural or recombinant mouse ifn g in serum, plasma, and cell culture supernatants. A monoclonal capture antibody specific for human ifn. Elisa max deluxe elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price. The human ifn alpha elisa is for research use only. Using the elisa protocol, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay human ifn. Detailed procedures for enzymelinked immunospot elispot and introduction to how elispot works. Elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant human ifn. A targetspecific antibody has been precoated in the wells of the supplied microplate. Human interferon alpha 1 elisa kit ab2479 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use.
Type i interferons induced by radiation therapy mediate. Activation of ifn gamma takes place through binding of ifn gamma receptor i and ii, and activating the jakstat pathway. Verikine human ifn beta elisa kit pbl assay science. Intended use quantiferontb gold qft is an in vitro diagnostic test using a peptide cocktail simulating esat6, cfp10, and tb7. Dilute the antigen to 12 ugml in coating solution 2. Elisa with streptavidin conjugated to horseradish peroxidase hrp. Interferon alpha human elisa kit thermo fisher scientific. Elisa max deluxe elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets. The ifn human elisa kit is designed to detect and quantify the level of human interferon alpha ifn in media using 96well plates and a microplate reader. The suggested diluent is suitable for the analysis of most cell culture supernate samples. The assay will exclusively recognize both natural and recombinant hu hu ifn principle of the method. Elisa kit this product is suitable for in vitro quantitative detection of mouse serum, plasma or cell culture supernatant and organizations in. Interferons ifns activity has been discovered due to their antiviral effects.
Quantitate human ifn gamma with 470 pgml sensitivity. The verikinetm mouse interferon beta elisa kit has been developed to quantify levels of ifn. Bowdish lab, mcmaster university hamilton, on, canada. Easytorun sandwich elisa has a total assay incubation time is only 2. Refer to the lotspecific c of a for amount supplied. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. This mouse ifn gamma uncoated elisa contains the necessary reagents, standards, buffers and diluents for performing quantitative enzymelinked immunosorbent assays elisa.
Elisa this antibody is immobilized to a microtiter plate. A userfriendly, highly sensitive assay to detect the ifn. Human ifn gamma high sensitivity elisa kit ab236895 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifn gamma protein in cit plasma, edta plasma, saliva, serum, and cell culture supernatant. Ifna interferonalpha elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. This duoset elisa development kit contains the basic components required for the development of sandwich elisas to measure natural and recombinant mouse ifn gamma. Sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price.
Dilute in pbs without carrier protein to the working. Use clean, dedicated reagent trays for dispensing the washing liquid, conjugate and substrate reagent. Cytokinespecific monoclonal capture antibodies are immobilized on an ethanoltreated pvdf membrane plate. Ifn alpha interferon alpha is a type i interferon belonging to a family of structurally related, speciesspecific proteins exclusive to vertebrates. Invitrogen ifn gamma human uncoated elisa kit with plates.
Uncoated elisa the following protocol is a general guideline for the uncoated sets materials provided please refer to the certificate. Elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse ifn. Use clean, dedicated reagent trays for dispensing the washing liquid. Add 100 ul of diluted antigen to appropriate wells. Ifn gmcsf the biomarker reference set for cancers in women brscw was provided by the national cancer institute on behalf of the early detection research network edrn. Human ifn alphabeta r2 elisa kit ifnar2 ab264610 abcam. The assay is based on the international reference standard for human interferon alpha hu ifn. In our plate coating guide you can find detailed information on how to perform. The following is a protocol that was developed for the tgf. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat ifn. Human ifn alpha interferon alpha elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. Human interferon beta ifn beta elisa kit elisa genie.
The enzymelinked immunospot elispot assay is a sensitive method for quantification of the number of cytokine secreting cells. Human ifn alpha platinum elisa thermo fisher scientific. Human ifn gamma elisa kit ab174443 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifngamma protein in human cell culture supernatant, plasma. Human interferon beta ifn beta elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 18. Ifn gamma does not show any homology with ifn alpha or. Sufficient materials are provided to yield approximately 20 plates of 96wells if the.
Mouse interferon gamma ifn elisa assay kit 410 catalog number. Elisa is an enzymelinked immunosorbent assay for the quantitative detection of human ifn the human ifn. Production find, read and cite all the research you need on researchgate. Mouse interferon alpha 2 elisa kit ab211648 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 2 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. Ifn gamma human elisa kit, high sensitivity invitrogen. The assay is based on the international reference standard for human interferon beta hu ifn. Opened reconstituted reagents standard sample diluent may be stored for up to 1 month at. The enzymelinked immunosorbent assay elisa is a frequently used application which determines the concentration of an analyte in solution. Ifn gamma is a proinflammatory cytokine secreted by t and nk cells in response to viral infection and other inflammatory conditions. Quantitate human ifn gamma with 2 pgml sensitivity. Pdf on jan 1, 2017, michelle wykes and others published elispot assay to measure peptidespecific ifn.
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